Electrical stimulation and millisecond-class ultralow temperature rapid freezing method and device thereof



The invention relates to the technical field of an electron microscope and ultralow temperature rapid freezing. The invention aims to provide a method and special equipment which aim to ensure a biological tissue to be rapidly frozen and fixed at an ultralow temperature at the moment that the biological tissue is simulated and are convenient to research the morphological change of an ultrastructure when physiological functions of the biological tissue are changed in various specific states. The equipment disclosed by the invention comprises a sample stage (3), a vertical sliding plate (5), an ultralow temperature freezing chamber (7), an infrared sensor A (9), an infrared sensor B (10), a timer (11), a programmable stimulator (14), a computer (15) and the like. The electrical stimulation and millisecond-class ultralow temperature rapid freezing method and device provided by the invention solves the problems of ensuring the morphological research to be synchronized with the moment physiological function change of the biological tissue and obtaining an ultrastructure morphological image, the ion concentration and the locus change of the biological tissue in real time; when the physiological functions of the biological tissue are changed, more realer ultrastructure information can be obtained; the physiological functions and the morphological research can be synchronously carried out on the level of the millisecond class; and the method and the special equipment have important significance for developing the subjects such as the electron microscopy, the cell ultrastructure morphology, the physiology, the pathology and the like.




Download Full PDF Version (Non-Commercial Use)

Patent Citations (1)

    Publication numberPublication dateAssigneeTitle
    CN-202614579-UDecember 19, 2012中国人民解放军第二军医大学Ultralow-temperature quick freezing device

NO-Patent Citations (3)

    张健伟等: "电刺激与冷冻固定同步以获得生物组织毫秒级功能变化时的超微结构", 《解剖学杂志》, vol. 29, no. 3, 31 December 2006 (2006-12-31), pages 362 - 363
    杨勇骥等: "骨骼肌在兴奋收缩偶联潜伏期内毫秒级功能变化时的形态研究", 《电子显微学报》, vol. 17, no. 4, 31 December 1998 (1998-12-31), pages 413 - 414
    杨勇骥等: "骨骼肌毫秒级功能变化时的结构研究", 《电子显微学保》, vol. 18, no. 5, 31 October 1999 (1999-10-31), pages 517 - 521

Cited By (1)

    Publication numberPublication dateAssigneeTitle
    WO-2013174147-A1November 28, 2013中国人民解放军第二军医大学电刺激—毫秒级超低温快速冷冻方法及其装置