Electrical stimulation and millisecond-class ultralow temperature rapid freezing method and device thereof

电刺激—毫秒级超低温快速冷冻方法及其装置

Abstract

The invention relates to the technical field of an electron microscope and ultralow temperature rapid freezing. The invention aims to provide a method and special equipment which aim to ensure a biological tissue to be rapidly frozen and fixed at an ultralow temperature at the moment that the biological tissue is simulated and are convenient to research the morphological change of an ultrastructure when physiological functions of the biological tissue are changed in various specific states. The equipment disclosed by the invention comprises a sample stage (3), a vertical sliding plate (5), an ultralow temperature freezing chamber (7), an infrared sensor A (9), an infrared sensor B (10), a timer (11), a programmable stimulator (14), a computer (15) and the like. The electrical stimulation and millisecond-class ultralow temperature rapid freezing method and device provided by the invention solves the problems of ensuring the morphological research to be synchronized with the moment physiological function change of the biological tissue and obtaining an ultrastructure morphological image, the ion concentration and the locus change of the biological tissue in real time; when the physiological functions of the biological tissue are changed, more realer ultrastructure information can be obtained; the physiological functions and the morphological research can be synchronously carried out on the level of the millisecond class; and the method and the special equipment have important significance for developing the subjects such as the electron microscopy, the cell ultrastructure morphology, the physiology, the pathology and the like.
本发明涉及电镜技术和超低温快速冷冻技术领域,本发明的目的在于提供一种为了使生物组织在受到刺激的瞬间即被超低温快速冷冻固定,便于研究该生物组织各种特定状态下生理功能变化时的超微结构形态变化的方法和专用设备。本发明的设备包括:样品台(3)、垂直滑板(5)、超低温冷冻室(7)、红外传感器A(9)、红外传感器B(10)、计时器(11)、程控刺激器(14)和计算机(15)等。本发明解决与生物组织瞬时生理功能变化同步,实时获得其超微结构形态像及离子浓度、位点变化的问题,将能获得生物组织生理功能变化时,更真实、更多的超微结构信息,使生理功能与形态学研究能在毫秒级的水平上同步进行,将对电子显微镜学、细胞超微结构形态学、生理学及病理学等学科的发展有重要意义。

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Patent Citations (1)

    Publication numberPublication dateAssigneeTitle
    CN-202614579-UDecember 19, 2012中国人民解放军第二军医大学Ultralow-temperature quick freezing device

NO-Patent Citations (3)

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Cited By (1)

    Publication numberPublication dateAssigneeTitle
    WO-2013174147-A1November 28, 2013中国人民解放军第二军医大学电刺激—毫秒级超低温快速冷冻方法及其装置