从干燥的生物流体样品获取分析物的设备和方法

Apparatus and methods for acquiring analytes from dried biological fluid sample

Abstract

An apparatus and methods for acquiring analytes from a dried biological fluid sample are provided. The apparatus for acquiring the analytes from the dried biological fluid sample includes a tube, a substrate in a proximal section of the tube, and a sorbent bed in a distal section of the tube. A biological fluid sample is dispensed on the substrate and is dried to form a dried sample. A conditioning solvent is flowed into the distal section to condition the sorbent bed. A first elution solvent is flowed through the substrate and the sorbent bed. Analytes are eluted from the dried sample and retained on the sorbent bed. A second elution solvent is flowed through the substrate and the sorbent bed. The analytes are eluted from the sorbent bed, pass through an opening, and are collected. Alternatively, an elution solvent is flowed through the substrate and the sorbent bed such that analytes eluted from the dried sample pass through the sorbent bed and the opening for collection, while non-analytes are retained on the sorbent bed.
本发明涉及从干燥的生物流体样品获取分析物的设备和方法,用于从干燥生物流体样品获取分析物的设备包括管、管的近端段中的基材以及管的远端段中的吸附剂床。生物流体样品被分配在基材上并干燥以形成干燥样品。使调节溶剂流入远端段中,以调节吸附剂床。使第一洗脱溶剂流动通过基材和吸附剂床。分析物被从干燥样品洗脱,并保留在吸附剂床上。使第二洗脱溶剂流动通过基材和吸附剂床。分析物被从吸附剂床洗脱,通过开口并被收集。或者,使洗脱溶剂流动通过基材和吸附剂床,使从干燥样品洗脱的分析物通过吸附剂床和开口用于收集,而非分析物被保留在吸附剂床上。

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